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Description
Accurate amino acid composition analysis for HPLC, UHPLC, and UPLC
Accurately separating, identifying, and quantitating amino acids is challenging. The amino acid sequence is the primary structure of a protein and it is unique to a particular peptide or protein. Amino acids are also key ingredients in the cell culture media and feed used to prepare recombinant proteins. Determining amino acid composition in protein/peptide hydrolysates, foods, feeds, and pharmaceutical preparations is important, as regularly monitoring individual amino acid consumption can help you optimize the conditions in the bioreactor.
You can easily analyze amino acids with the many manual and automated solutions that use pre-column derivatization from Waters. With Waters AccQ•Tag Ultra UPLC/UHPLC reagent kit and AccQ•Fluor HPLC reagent kit, you’ll have what your lab needs for accurate amino acid composition analysis.
AccQ•Tag Ultra and AccQ•Fluor derivatization solutions utilize a single reagent to tag both primary and secondary amino acids, reducing reagent usage by 50% compared to competitive kits that require two separate derivatization compounds. This streamlined approach simplifies the gradient and eliminates the need to switch detection wavelengths mid-method.
Overview
Achieve stable derivatives with simple, fast, sensitive pre-column derivation using UPLC, UHPLC, or HPLC instrumentation
Derivatized amino acids resolved and quantified with UV and Fluorescence
Complete workflow solution including derivatization reagents, columns, eluents, standards, and methods
Automation enabled on multiple platforms with verified scripts, including Andrew+ Robot and OneLab protocols
Application-specific amino acid standard kits for identification and quantification
