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Description
Faster, more efficient online protein digestion
Fast, efficient, and reproducible online pepsin digestion is critical in experiments that require more peptide coverage. With the Waters Enzymate BEH Pepsin Column, you can easily incorporate online protein digestion into existing HDX- MS workflows.
The Enzymate BEH Pepsin Column efficiently digests intact proteins into peptides in an online HDX system, typically in about 30 seconds. The peptic peptides are then retained on a trapping column and refocused onto a sub-2-μm ACQUITY UPLC Column for elution into high-resolution mass spectrometry.
Overview
Achieve fast, reproducible, and efficient online pepsin protein digestion, typically within 30 seconds
Shorten overall protein sample preparation time
Optimize protein digestion efficiency by changing temperature and/or flow rate
Easily connect to the ACQUITY UPLC M-Class with HDX Technology to characterize sample protein
Recommended Use: For the online digestion of intact proteins into peptides.
